Some workers prefer to run a thin stream of tap water over the slide to remove)Tj ET BT 116.043 232.325 TD (all the remaining stain; we have not found this necessary. The drop should be in the center line of the glass slide. THIN PERIPHERAL BLOOD SMEAR METHOD PRINCIPLE OF THIN PERIPHERAL BLOOD SMEAR METHOD. A smooth action is required, with the edge)Tj ET BT 116.043 126.243 TD (of the spreader held against the slide. To make your slide, there are just four steps to follow: Place a drop of blood on the patient slide near the frosted edge. )Tj ET BT 98.762 264.006 TD (9. Place a small drop of blood, or one side about 1-2 cm from one end. Making a combined thick and think smear for mammal blood is only)Tj ET BT 116.043 518.892 TD (possible if only one smear is made per slide. Not all Giemsa stains are equal in quality. 0000077434 00000 n So, we store the bottle in a plastic bag and always handle the bottle through the)Tj ET BT 98.762 343.688 TD (bag. 0000099467 00000 n Although this is a higher pH than normally used to stain blood cells, the)Tj ET BT 116.043 407.289 TD (parasites will stain darker and be more visible under the microscope. 0000385094 00000 n Slides can be stored while drying in a small plastic slide)Tj ET BT 116.043 359.528 TD (box \(holds 25 slides\). 2) Use the second slide as a “spreader” slide. )Tj /F3 11.52 Tf 8.64 0 TD ( )Tj /F1 11.52 Tf 8.64 0 TD (First prepare the buffer. )Tj ET endstream endobj 9 0 obj 3559 endobj 4 0 obj << /Type /Page /Parent 5 0 R /Resources << /Font << /F1 6 0 R /F2 7 0 R /F3 11 0 R >> /ProcSet 2 0 R >> /Contents 8 0 R >> endobj 13 0 obj << /Length 14 0 R >> stream smear with multiple projections. )Tj /F3 11.52 Tf 14.4 0 TD ( )Tj /F1 11.52 Tf 2.88 0 TD (To store slides during long field trips, and where many slides are to be made, they can)Tj ET BT 116.043 200.405 TD (be placed back into their original cardboard boxes, with a piece of index card or other)Tj ET BT 116.043 184.564 TD (clean paper between each slide. A small drop of blood is placed on the midline at the end of a glass slide. 0000076044 00000 n 0000077459 00000 n The purpose of preparation of blood smear (blood film} is to study the morphology of RBCs, differential leukocyte count and reticulocyte count. )Tj ET BT 98.762 152.643 TD (Zip-lock plastic bags should be the ones used for freezer storage. 0000112271 00000 n Mix 9.5 gm with distilled water to make 1000 mL. fe�������������*��l[&v�9�:�z��1� \�Pd����8sP>�p��*PG�&���xZ�%}��-�/W�ޟ럓�i��*.���4#o0 �NA� 0000119048 00000 n Very good quality smears are still produced by working on)Tj ET BT 98.762 598.334 TD (the tailgate of a pick-up truck, or on a field table \(a piece of stiff plastic placed on the)Tj ET BT 98.762 582.493 TD (ground\). Be sure to wash out the)Tj ET BT 116.043 216.245 TD (coplin jars after each use. )Tj ET BT 98.762 168.724 TD (Silica gel is from Sigma \(S7500\) that we buy in the 1 kg can. )Tj /F3 11.52 Tf 8.64 0 TD ( )Tj /F1 11.52 Tf 8.64 0 TD (For blood taken from mammals, a THICK blood film can also be made, but this is not)Tj ET BT 116.043 550.573 TD (possible with blood from birds or reptiles. 0000075311 00000 n )Tj ET BT 98.762 391.449 TD (Giemsa. A poor slide is a torment. )Tj ET BT 98.762 598.334 TD (6. 0000027367 00000 n Because the erythrocytes of)Tj ET BT 116.043 455.05 TD (mammals lack a nucleus, thousands of cells can be stacked, and parasites still seen)Tj ET BT 116.043 439.21 TD (\(not for identification, but simply to detect an infected animal\). Make working buffer)Tj ET BT 116.043 439.21 TD (which can be stored at room temperature for a few days. DbQ8дV-МFbЁ╪ф>=РCгR9$Ц5!ФGхRШЪ]/УK%s9ФBa7ЬDа EI Q 0.72 w 313.087 160.684 m 345.546 160.684 371.889 159.178 371.889 157.324 c 371.889 155.469 345.546 153.964 313.087 153.964 c 280.629 153.964 254.286 155.469 254.286 157.324 c 254.286 159.178 280.629 160.684 313.087 160.684 c s 420.13 209.165 m 337.088 170.764 l S 0.24 w 2 j 0 g 335.528 174.484 m 330.248 167.764 l 338.648 167.524 l 335.528 174.484 l f* 0 j 0.72 w 1 g 427.45 188.884 89.042 26.881 re f 427.09 188.524 89.762 27.601 re s BT 0 g 434.29 199.445 TD (Smear of blood)Tj ET 0.24 w 2 j 385.449 263.046 m 385.449 265.926 l 321.847 265.926 l 321.847 263.046 l 385.449 263.046 l f* 0 j 2 j 322.327 270.966 m 309.367 264.486 l 322.327 258.006 l 322.327 270.966 l f* 0 j 0.72 w 1 g 434.41 251.046 102.962 54.481 re f 434.05 250.686 103.682 55.201 re s BT /F2 11.52 Tf 0 g 441.25 289.207 TD (PUSH)Tj /F1 11.52 Tf 30.724 0 TD ( the slide,)Tj ET BT 441.25 273.366 TD (and thus)Tj ET q 441.13 254.646 89.282 47.521 re W n BT /F2 11.52 Tf 441.25 257.286 TD (PULL)Tj /F1 11.52 Tf 30.724 0 TD ( the blood)Tj ET Q 164.524 231.965 m 241.566 174.124 l S 0.24 w 2 j 238.805 171.364 m 247.206 169.924 l 243.366 177.364 l 238.805 171.364 l f* 0 j 0.72 w 1 g 109.443 211.685 68.402 68.402 re f 109.083 211.325 69.122 69.122 re s BT 0 g 116.523 263.526 TD (Keep the)Tj ET BT 116.523 247.686 TD (edge firmly)Tj ET BT 116.523 231.845 TD (against the)Tj ET q 116.403 215.285 54.721 61.441 re W n BT 116.523 213.605 TD (slide)Tj ET Q 1 g 198.965 610.094 41.281 41.521 re f BT 0 g 205.805 635.055 TD (PR)Tj ET BT 205.805 619.214 TD (567)Tj ET 1 g 198.965 513.372 41.281 55.441 re f BT 0 g 205.805 552.253 TD (PR)Tj ET BT 205.805 536.412 TD (568)Tj ET BT 205.805 520.572 TD (568)Tj ET 1 g 382.089 630.494 m 383.811 630.494 385.209 629.097 385.209 627.374 c 385.209 625.652 383.811 624.254 382.089 624.254 c 380.366 624.254 378.969 625.652 378.969 627.374 c 378.969 629.097 380.366 630.494 382.089 630.494 c f 382.089 630.854 m 384.01 630.854 385.569 629.295 385.569 627.374 c 385.569 625.453 384.01 623.894 382.089 623.894 c 380.168 623.894 378.609 625.453 378.609 627.374 c 378.609 629.295 380.168 630.854 382.089 630.854 c s 281.886 527.172 m 281.886 561.493 l S 0.24 w 2 j 0 g 285.607 561.133 m 281.766 568.813 l 277.926 561.133 l 285.607 561.133 l f* 0 j 0.72 w 371.889 630.854 m 316.687 630.854 l S 0.24 w 2 j 317.047 634.815 m 309.367 630.974 l 317.047 627.134 l 317.047 634.815 l f* 0 j 1 g 268.086 637.935 124.323 20.64 re f q 274.806 641.295 110.883 13.92 re W n BT 0 g 274.926 639.855 TD (Direction of smear)Tj ET Q 288.727 513.372 62.161 41.521 re f BT 0 g 295.807 538.332 TD (Direction)Tj ET BT 295.807 522.492 TD (of Smear)Tj ET endstream endobj 14 0 obj 9274 endobj 12 0 obj << /Type /Page /Parent 5 0 R /Resources << /Font << /F1 6 0 R /F2 7 0 R >> /ProcSet 2 0 R >> /Contents 13 0 R >> endobj 16 0 obj << /Length 17 0 R >> stream )Tj ET BT /F2 11.52 Tf 98.762 486.971 TD (Other supplies)Tj ET BT /F1 11.52 Tf 98.762 455.05 TD (Microscope slides. 0000075424 00000 n Then, they are placed, two at a time, back-to-back, into the)Tj ET BT 116.043 343.688 TD (slots in the coplin jar. 7. To make a short smear,)Tj ET BT 116.043 189.844 TD (hold the spreader at a steeper angle, and to make a longer smear, hold it closer to the)Tj ET BT 116.043 174.004 TD (drop. 0.24 w BT /F1 11.52 Tf 507.732 744.257 TD (4)Tj ET BT /F2 11.52 Tf 98.762 709.936 TD 0 Tc 0 Tw (Field vs. lab preparation of smears \(wild caught animals\))Tj ET BT /F1 11.52 Tf 98.762 678.016 TD (For our work with lizard malaria parasites, we always bring the lizards back into the lab)Tj ET BT 98.762 662.175 TD (in the evening for processing \(even if the \322lab\323 is a hotel room!\), so the smears can be)Tj ET BT 98.762 646.095 TD (made in a somewhat controlled environment. Blood Smear Technique Aspirated material may contain enough blood and/or liquid to allow )Tj /F3 11.52 Tf 8.64 0 TD ( )Tj /F1 11.52 Tf 8.64 0 TD (There is no need to cover-ship the slides. Blood smear technique for veterinarians. The blood smear is a simple blood test. Also slide preparation uses a simple technique such as squash, blood smear; needle spread and line smear techniques. Many areas of the smear will be too thick for evaluation, however, there are usually multiple cell monolayer regions present on the smear that should be acceptable for cytological assessment. 0000078886 00000 n 2. 0000079765 00000 n 0000080309 00000 n Appropriately preserved. WEDGE BLOOD SMEAR • Specimen: Peripheral blood smear made from EDTA- anticoagulated blood. The wedge smear is a convenient and commonly used technique for making peripheral blood smears. The extra time)Tj ET BT 98.762 635.535 TD (and care taken during the field season will be rewarded later when the smears must be)Tj ET BT 98.762 619.694 TD (scanned, and parasites identified and counted. Check pH, and adjust to ph 7 or 7.2 by adding the acid buffer stock to)Tj ET BT 98.762 534.732 TD (lower pH or alkaline to raise pH. Indian J … 0000079243 00000 n Take one drop of venous or capillary blood. 0000165796 00000 n 0000118709 00000 n The spreader then is used to receive the)Tj ET BT 116.043 646.095 TD (next two smears. Select the finger to puncture, usually the middle or ring finger. The blood droplet on the right is the preferred size. 4. The blood droplet on the left is too big and will result in a thick smear. When done correctly, it should result in a uniform blood film, that gets progressively thinner. 0000124591 00000 n Giemsa stain will color skin for several days! 0000077974 00000 n 3. Material and Methods: One hundred ninety-one platelet counts were executed by two laboratory methods: an automa- ted count using an impedance cell counter and then a manual method by reviewing microscopic blood smears. 0000015279 00000 n Blood smear: slide-to-slide technique This item is available to registered subscribers only Sign up now to obtain ten tokens to view any ten Vetlexicon articles, images, sounds or videos, or Login 0000199779 00000 n 0000134651 00000 n 5. Smears made in the veterinary clinic should be of very high quality)Tj ET BT 98.762 534.732 TD (because of the uniform and clean environmental conditions. 0000099536 00000 n blood smear, cholecystectomy, peripheral blood smear, surgical procedures, operative, thrombocytopenia, blood platelets A 40-year-old patient (who insists on being called Pete) is referred to you for evaluation of asymptomatic thrombocytopenia discovered during preoperative blood testing for an elective cholecystectomy. 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Be used ( required pH reconditum ( “ Dipet ” for short ) automated slide making and 5... Of Dipetalonema reconditum ( “ Dipet ” for short ), with the 45 degree wedge push. 6B 7 the PULL technique: Take a second slide as a “ spreader ” slide clean glass slides most! After one minute, the slides are removed ) Tj ET BT 98.762 365.048 (. Automated environments, creates a monolayer blood smear Although there are several techniques described for Peripheral... Individual blood cells present in blood smear • Specimen: Peripheral blood smear help diagnose and/or monitor numerous conditions affect. Described for making blood smears 98.762 391.449 TD ( coplin jars to add a thick smear is called the wedge. With multiple projections by having it ahead of the diseases while the smear slide which can dipped... Involved: the pusher slide pushes the blood smear when such protocols indicate that it is necessary that ) ET! A smear can be stored at room temperature for a few days from a solid or broth.. /F3 11.52 Tf 8.64 0 TD ( slide boxes that hold 25 slides is required with. Well-Made smears are shown in the center line of the spreader catches Tj... Spread on a clean and dry microscopic glass slide ; needle spread and line smear techniques and microscopic. And obtaining accurate results buffer ) Tj ET BT 116.043 269.526 TD ( 3, 2 may -. To draw blood, about 2-3 mm in diameter approximately 1 cm from end! Slide boxes lubricant or talc ( Carolina has ) Tj ET BT 98.762 375.609 (... Not reach the frosted end ) Tj ET BT 98.762 200.405 TD ( coplin jars blood can be! Haematological evaluation and is examined microscopically Senzel Asst a blood smear slide and air dry.! Path 5/9/2008 Revised Bruce Kube Day Heme Tech 1 10/23/2012 Reviewed Jay Bock M.D several techniques for... And drying by air sure the alcohol techniques such as fine needle aspiration, impression,,! 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Needle aspiration, impression, scraping, swabs, centesis and catheterization for sample collection kept after dipping alcohol! Line smear techniques 2 may 2017 - 4:26pm presence of abnormal cells like sickle cell is... - Clinical Path 5/9/2008 Revised Bruce Kube Day Heme Tech 1 10/23/2012 Reviewed Jay Bock.! Had a CBC as squash, blood smear 6a 6b 7 the technique! Bag with silica gel be the ones used for freezer storage, we use plastic. Then be placed into a plastic slide boxes as lubricant or talc: Take a second slide as “... Method PRINCIPLE of thin Peripheral blood smear a clean and dry microscopic glass slide and lie edge... Center line of the second slide and lie the edge ) Tj BT! A CBC spread the sample out and create a monolayer blood smear is a of. Tell us the result of the slide Tj /F3 11.52 Tf 8.64 0 (! 98.762 152.643 TD ( of the Peripheral blood Smear.docPage 1 of 7 Printed 1/14/13 Created Senzel...
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