Pour 1Tbsp of cabbage juice into each of the cups, swirling gently. You can try seeing how tap water affects pH by creating one batch of anthocyanin indicator using distilled water, and another batch with tap water. Red Cabbage as a pH Indicator Making the pH Indicator What You Need: • A red cabbage • A pot of water Procedure: 1. indicator to the same test tube. The pH of pure water is 7 and the normal range of surface water is between 6.5 and 8.5. The juice is prepared by soaking chopped red cabbage in boiling water for at least 10 minutes and then filtering the solution to eliminate any plant material. (This should provide you with 600-800 ml of cabbage juice.) In this case, the chemicals with indicator properties in the cabbage leaves are dissolved in boiling water.] The Cabbage Indicator Experiment My hypothesis for this experiment is if I put an acid or a base in to a neutral then it will change colors. Leave to cool for about 30 minutes. The cabbage contains a natural pH indicator that changes colors according to the acidity of the solution. The use of anthocyanins in red cabbage extracts as pH indicators has long been a popular classroom activity. Making the pH Indicator (this can be done in advance): 1. 2. My hypothesis was correct in assuming that it would change colors with different substances or mixtures. Add a small amount of each test substance to a separate cup or container, try to keep the amount of test substance the same. Record the color and pH under Buffer for the “Original (Before HCl added)” row. Q8. Testing pH… Close the top and let it blend at high power for 30 seconds. 5. 6. It contains water-soluble anthocyanins, which have indicator properties and vivid colors, making it easy to visualize color changes that occur when they are exposed to different pH conditions. This investigation explores further applications of plant-derived dyes including reversible reactions based on oxidation/reduction chemistry and other reactions to illustrate colour changes that are Distilled water is more likely to have a neutral pH. Break apart the cabbage into small pieces and place into a pot 2. [Note: A solution in this sense simply means a substance dissolved in water. Put the red cabbage leaves into the blender with 800mL of water. Next, use the water bottle to add about 1 mL (about 15 drops) of deionized water into another clean small test tube, then add 5-10 drops of cabbage indicator. 2. Liquid: Color Predicted pH Actual pH Water Lemon Juice Baking Soda Shampoo Red cabbage juice is a good indicator of pH because it contains flavin, a pigment molecule that changes colors according to the concentration of hydrogen ions in a solution. Cover the cabbage in water and boil for 20 minutes, allowing the color to leach out of the cabbage (see picture on right). Write down what color each solution turns the cabbage juice, based on the following pH scale. Are the colors the same? Brassica oleracea, more commonly known as red cabbage, is useful as an acid-base indicator owing to the fairly wide range of pH related color changes. The indicator paper works in the same way as the indicator solution itself: the paper changes color when it comes in contact with an acidic or basic substance. 3. Since the anthocyanin indicator needs to have a pH as close to neutral as possible, this is important. In this experiment, we will use the juice from red cabbage as a pH indicator to test common household liquids and determine their pH levels. Sieve the water and cabbage into a jug – you will notice that the cabbage liquid is very purple in colour. Pour the mixture through a strainer into a large container. 3. For baking soda, add 1 tsp of baking soda into 2 tsps water. Flowers, fruits and vegetables contain a diverse range of anthocyanins. 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